Wie CYPs Medikamente verarbeiten: Einblicke durch innovative Labortechniken

Cytochrome-P450 enzymes (CYPs) represent a major class of enzymes responsible for the metabolism of xenobiotic substances. To assess the role of CYPs in drug metabolism, model systems such as liver cells are used but they harbor a variety of CYPs. Mono-CYP systems were established with a defi ned CYP activity to study the underlying mechanisms. We utilize a Mono-CYP system based with cell-free protein synthesis. We have shown that this combination enhances the understanding of CYP reactions.