• English
  • Deutsch
  • Log In
    Password Login
    Research Outputs
    Fundings & Projects
    Researchers
    Institutes
    Statistics
Repository logo
Fraunhofer-Gesellschaft
  1. Home
  2. Fraunhofer-Gesellschaft
  3. Anderes
  4. Marmoset monkeys (Callithrix jacchus) develop eosinophilic airway inflammation after house dust mite exposure
 
  • Details
  • Full
Options
2020
Paper (Preprint, Research Paper, Review Paper, White Paper, etc.)
Title

Marmoset monkeys (Callithrix jacchus) develop eosinophilic airway inflammation after house dust mite exposure

Title Supplement
Preprint Published on bioRxiv
Abstract
Background: Extensive analysis of eosinophilic airway inflammation in human-relevant animal models is required to test novel, human-specific pharmaceuticals. This requires species, which show high genetic homology to humans such as non-human primates. Efficacy assessment of novel human-specific biologicals in eosinophilic airway inflammation is currently performed in the cost-intensive macaque asthma model. Objective: The present study investigated whether marmoset monkeys (Callithrix jacchus), a small-bodied non-human primate species from the New World, develop eosinophilic airway inflammation in response to house dust mite allergen exposure (HDM, Dermatophagoides pteronyssinus). Methods: Marmoset monkeys were sensitized against HDM by subcutaneous (s.c.) injection and subsequent intratracheal (i.t.) HDM aerosol challenges. Airway and systemic immunologic reactions were monitored and sensitivity towards glucocorticoid therapy was assessed. The pulmonary immunologic response was analyzed by repetitive bronchoalveolar lavage (BAL). Results: Bronchoalveolar lavage fluid (BALF) exhibited increased levels of eosinophils, mast cells, and lymphocytes, as well as interleukin (IL)-13 after HDM challenges, compared to negative controls. The systemic immunologic response was assessed in peripheral blood mononuclear cells derived from sensitized animals, which secreted increased IL-13 and IFN-γ upon allergen stimulation in contrast to non-sensitized negative control animals. Although IgE was not detectable, HDM-specific serum IgG was elevated in sensitized animals. Both airway and systemic responses were reduced by treatment with glucocorticoids. However, lung function and pathological analyses did not reveal significant differences between groups. Conclusion: In conclusion, marmoset monkeys developed a mild HDM-induced eosinophilic airway inflammation useful for efficacy testing of novel human-specific biologicals.
Author(s)
Dahlmann, Franziska  
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Curths, C.
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Wichmann, J.
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Jimenez Delgado, S.
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Twisterling, E.
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Kap, Y.S.
sl-0
Driel, N. van
sl-0
Hart, BA’t
sl-0
Knauf, Y.
sl-0
Becker, T.
sl-0
Dunker, Sarah  
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Windt, Horst  
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Bleyer, M.
sl-0
Kaup, F.J.
sl-0
Sewald, Katherina  
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Braun, Armin  
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Knauf, S.
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
Open Access
DOI
10.1101/2020.02.24.962498
Additional full text version
Landing Page
Language
English
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
  • Cookie settings
  • Imprint
  • Privacy policy
  • Api
  • Contact
© 2024