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  4. Enhanced Fluorescence Detection of Interleukin 10 by Means of 1D Photonic Crystals
 
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2021
Journal Article
Title

Enhanced Fluorescence Detection of Interleukin 10 by Means of 1D Photonic Crystals

Abstract
In the present communication, we report on the exploitation of a Bloch surface wave-enhanced fluorescence scheme for the detection of Interleukin (IL)-10 in a protein-rich buffer mimicking a biological sample. IL-10 is a cytokine known for its potent anti-inflammatory and immunosuppressive effects. It is considered a valuable biomarker for prognostic prediction for both solid tumors and hematological malignancies, and recently, a distinguishing feature of hyperinflammation during severe viral infections. To demonstrate the validity of the technique, we transferred all the reagents and working concentrations used in a gold-standard technique, such as ELISA, to our assay, with a substantial reduction in the execution time and without using any enzymatic amplification during IL-10 recognition. We estimate a limit of detection (LoD) in terms of the concentration of IL-10 in solution of the order of 110 pg/mL (5.8 pM) with a 14% accuracy; in other terms, the presented technique is compatible with the assay range and resolution (1.6 pM) of commercial gold-standard ELISA kits. Moreover, such LoD successfully matches the concentrations reported in literature for IL-10 detection in COVID-19 patients, making the BSW-based sensors a viable solution for rapid and accurate screening of COVID-19-related molecules.
Author(s)
Occhicone, A.
Porto, P. Del
Danz, N.
Munzert, P.
Sinibaldi, A.
Michelotti, F.
Journal
Crystals  
Open Access
DOI
10.3390/cryst11121517
Additional link
Full text
Language
English
Fraunhofer-Institut für Angewandte Optik und Feinmechanik IOF  
Keyword(s)
  • Bloch surface waves

  • fluorescence

  • Interleukin-10

  • one-dimensional photonic crystals

  • sensors

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