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  4. Application of diffractive optical elements in confocal microscopy
 
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2019
Conference Paper
Title

Application of diffractive optical elements in confocal microscopy

Abstract
In traditional confocal microscopy, there is a tradeoff between the spatial resolution and the field of view due to the limitations of the objective lenses. To solve this problem, diffractive optical elements (DOEs) are used to generate illumination spots with high NA in a large area simultaneously. However, such DOEs in current research are only used as illuminators. In this work, the idea of superposition is utilized in DOEs to have flexible functionality and replace high-NA objectives for confocal measurement. To design the DOEs, different numerical simulation methods for light propagation are investigated and compared. Rayleigh-Sommerfeld integral is chosen to simulate the DOEs to get accurate results. Two kinds of DOEs are designed and simulated for 3D confocal surface measurements.
Author(s)
Li, Zheng  
Mainwork
Proceedings of the 2018 Joint Workshop of Fraunhofer IOSB and Institute for Anthropomatics, Vision and Fusion Laboratory  
Conference
Fraunhofer Institute of Optronics, System Technologies and Image Exploitation and Institute for Anthropomatics, Vision and Fusion Laboratory (Joint Workshop) 2018  
DOI
10.24406/publica-fhg-404882
File(s)
N-552191.pdf (15.56 MB)
Rights
Under Copyright
Language
English
Fraunhofer-Institut für Optronik, Systemtechnik und Bildauswertung IOSB  
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