Design, assembly and characterization of a humanized scFv framework library
Presentation held at the Bioforum Lüttich, 3 May 2002
The murine mAb Guy 13 binds to the surface protein SAI/II of S.mutans, the major cause of dental caries. Used in topical immunotherapy this mAb prevents recolonisation of the tooth surfaces by S.mutans and mediates a lasting protection from dental caries. Humanisation of Guy reduce the immunogenicity of the antibody in clinical applications to avoid negative side effects and eventually increase stability. The shown data successfully prove the concept of creating a CDR grafted framework library for humanization of a murine antibody, by design, assembly, cloning and characterization of a hybrid humanizedVL - mouseVH library. In the following the humanized VH library will be assembled and joined with the VL library to select humanized scFv's with optimal binding characteristics and a high number of human residues. Biosassays will follow to show the activity and immunogenic potential of the humanized molecules.