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  4. Method and kit for determining the genome integrity and/or the quality of a library of DNA-sequences otained by deterministic restriction site whole genome amplification
 
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Patent
Title

Method and kit for determining the genome integrity and/or the quality of a library of DNA-sequences otained by deterministic restriction site whole genome amplification

Abstract
The present invention relates to a method for determining the integrity of the genome of a sample and/or the quality of a library of DNA sequences obtained by deterministic restriction site whole genome amplification (DRS-WGA) of the genome of the sample comprising the steps of: (a) providing the library of DNA sequences; (b) amplifying the library of DNA sequences by PCR using at least one first primer pair which hybridises to a DNA sequence of the library having a length from 1000 bp to 5000 bp and corresponding to a sequence of the genome located on a first chromosome arm, the step of amplifying giving rise to a first PCR product from 50 to 1000 bp; (c) detecting the first PCR product; (d) correlating the presence of the first PCR product with the integrity of the genome and/or the quality of the library of DNA sequences. The present invention further relates to a related kit and uses thereof.
Inventor(s)
Klein, Christoph Andreas  
Polzer, Bernhard Michael  
Manaresi, Nicolo
Link to Espacenet
http://worldwide.espacenet.com/publicationDetails/biblio?DB=worldwide.espacenet.com&locale=en_EP&FT=D&CC=EP&NR=2881739A1
Patent Number
20130195770
Publication Date
2015
Language
English
Fraunhofer-Institut für Toxikologie und Experimentelle Medizin ITEM  
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