Bioreaktorsystem

(A1) Die Erfindung betrifft Bioreaktoren zur Kultivierung von Zellen, insbesondere von adhaerenten Zellen, die Verwendung der Bioreaktoren, insbesondere zur Kultivierung und Vermehrung von Zellkulturen, und Verfahren zur Kultivierung von Zellen unter Verwendung der erfindungsgemaessen Bioreaktoren. Ein besonderes Anwendungsgebiet ist die Verwendung der Bioreaktoren bei der GMP-konformen vollautomatischen Kultivierung und Vermehrung von Zellen.

DE 102009022354 A1 UPAB: 20101130 NOVELTY - The box- or cup-shaped bioreactor comprises reactor region, which is subdivided into two reactor areas through a membrane-unit fluid-permeable in part areas, and electrodes for transendothelial electrical resistance-value-measurement. The two reactor areas comprise an opening (24, 34) suited for introducing and/or discharging a fluid. The bioreactor is demountable in three parts such as a first reactor housing part that surrounds the first reactor area, a second reactor housing part that surrounds the second reactor area, and membrane unit fluid-permeable in part areas. DETAILED DESCRIPTION - The box- or cup-shaped bioreactor comprises reactor region, which is subdivided into two reactor areas through a membrane-unit fluid-permeable in part areas, and electrodes for transendothelial electrical resistance-value-measurement. The two reactor areas comprise an opening (24, 34) suited for introducing and/or discharging a fluid. The bioreactor is demountable in three parts such as a first reactor housing part that surrounds the first reactor area, a second reactor housing part that surrounds the second reactor area, and membrane unit fluid-permeable in part areas. The first reactor housing part is arranged as a reactor cover and the second reactor housing part is arranged as a reactor lower part. The reactor housing parts are connected to each other in air-tight manner through a screw joint. The membrane unit consists of a membrane (10) and a frame or the membrane unit consists of a membrane. The membrane unit is disc-shaped and lies between both membrane areas horizontal with its both surfaces. The membrane takes 25-100% of the surface of the membrane unit. The membrane has a pore size of 0.1-20 mu m, and a pore density of 107 pores per cm2. The first reactor housing part comprises an aeration opening equipped with a sterile filter, and/or a lockable pipette opening. An INDEPENDENT CLAIM is included for a method for cultivating cells. USE - Box- or cup-shaped bioreactor useful for cultivating cells (claimed). ADVANTAGE - The bioreactor is capable of simply cultivating the cells without any damage.