Verfahren und Vorrichtung zur Bildung einer dreidimensionalen Anordnung biologischer Zellen

(A1) Es wird ein Verfahren zur Bildung einer dreidimensionalen Zellanordnung (1) biologischer Zellen mit den Schritten Bereitstellung der Zellanordnung (1) auf einem biegsamen Substrat (10) und Deformation des Substrats (10) beschrieben, wobei die Deformation des Substrats (10) durch eine von den Zellen (2) auf das Substrat (10) ausgeuebte Attraktionskraft bewirkt wird. Es wird auch ein Substrat (10), das aus einem biegsamen Material gebildet ist und eine Substratoberflaeche (11) zur Anhaftung einer Zellanordnung (1) biologischer Zellen aufweist, beschrieben, wobei die Substratoberflaeche (11) eine Vielzahl von Kraftangriffspunkten aufweist, die zur Ausuebung einer Attraktionskraft eingerichtet sind, die von den Zellen auf das Substrat (10) uebertragbar ist, und das Substrat (10) eine Nachgiebigkeit derart aufweist, dass das Substrat (10) unter der Wirkung der Attraktionskraft deformierbar ist.

WO 2008155028 A1 UPAB: 20090114 NOVELTY - The method for forming a three-dimensional cell arrangement (1) of biological cells, comprises forming the cell arrangement on a flexible substrate (10), deforming the substrate by bending the substrate or folding the substrate such a way that the cell arrangement forms a flat cell layer (3) bending in an area or a three dimensional cell cluster, bending the cells in the cell arrangement, introducing further cells to the cell arrangement, releasing and/or removing the substrate and connecting the deformed substrates. DETAILED DESCRIPTION - The method for forming a three-dimensional cell arrangement (1) of biological cells, comprises forming the cell arrangement on a flexible substrate (10), deforming the substrate by bending the substrate or folding the substrate such a way that the cell arrangement forms a flat cell layer (3) bending in an area or a three dimensional cell cluster, bending the cells in the cell arrangement, introducing further cells to the cell arrangement, releasing and/or removing the substrate and connecting the deformed substrates. The deformation of the substrate is brought by an attractive force exerted by the cells on the substrate. The cells are arranged on the substrate surface in adherent manner and form a closed cell layer. The directed deformation of the substrate is intended with a predetermined head deformation direction, which is determined through a predetermined geometric arrangement of deformation area and/or force application point of the substrate. The deformed substrate forms a cavity, a tube form or a ball form. The deformation of the substrate comprises shaping a flat substrate layer or substrate part, whose position and/or bias is changed relative to the substrate through the deformation. The substrate layer is shaped and/or separated from the remaining substrate. The deformation of the substrate and the formation of the cell arrangement are carried out during or after cultivating the cells. The additional deformation power acts on the substrate and has an external mechanical power and/or an internal reaction power in the material of the substrate. The substrate is formed out of plastic, metal and/or a reabsorbable material, has a closed substrate surface, a substrate surface interrupted by holes or depressions and a lattice structure and carries a modification layer. An INDEPENDENT CLAIM is included for a substrate out of flexible material for forming a three-dimensional cell arrangement of biological cells. USE - Method for forming a three-dimensional cell arrangement of biological cells for implantation or healing purposes. ADVANTAGE - The method ensures effective formation of three-dimensional cell arrangement of biological cells by sufficient provision of cells with nutrient.