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  4. Low-energy electron irradiation (LEEI) for the generation of inactivated bacterial vaccines
 
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2022
Book Article
Title

Low-energy electron irradiation (LEEI) for the generation of inactivated bacterial vaccines

Abstract
Vaccines consisting of whole inactivated bacteria (bacterins) are generated by incubation of the pathogen with chemicals. This is a time-consuming procedure which may lead to less immunogenic material, as critical antigenic structures can be altered by chemical modification. A promising alternative approach is low-energy electron irradiation (LEEI). Like other types of ionizing radiation, it mainly acts by destroying nucleic acids but causes less damage to structural components like proteins. As the electrons have a limited penetration depth, LEEI is currently used for sterilization of surfaces. The inactivation of pathogens in liquids requires irradiation of the culture in a thin film to ensure complete penetration. Here, we describe two approaches for the irradiation of bacterial suspensions in a research scale. After confirmation of inactivation, the material can be directly used for vaccination, without any purification steps.
Author(s)
Fertey, Jasmin  orcid-logo
Fraunhofer-Institut für Zelltherapie und Immunologie IZI  
Standfest, Bastian  
Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA  
Beckmann, Jana
Fraunhofer-Institut für Organische Elektronik, Elektronenstrahl- und Plasmatechnik FEP  
Thoma, Martin  
Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA  
Grunwald, Thomas  
Fraunhofer-Institut für Zelltherapie und Immunologie IZI  
Ulbert, Sebastian  
Fraunhofer-Institut für Zelltherapie und Immunologie IZI  
Mainwork
Bacterial vaccines. Methods and protocols  
DOI
10.1007/978-1-0716-1900-1_7
Language
English
Fraunhofer-Institut für Organische Elektronik, Elektronenstrahl- und Plasmatechnik FEP  
Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA  
Fraunhofer-Institut für Zelltherapie und Immunologie IZI  
Keyword(s)
  • bacterial inactivation

  • bacterial vaccine

  • Bacterin

  • electron beam

  • Niederenergetische Elektronenstrahlung

  • low-energy electron irradation

  • LEEI

  • Bakterien

  • Elektron

  • Impfstoff

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