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  4. Powering artificial enzymatic cascades with electrical energy
 
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2020
Zeitschriftenaufsatz
Titel

Powering artificial enzymatic cascades with electrical energy

Abstract
We have developed a scalable platform that employs electrolysis for an in vitro synthetic enzymatic cascade in a continuous flow reactor. Both H2 and O2 were produced by electrolysis and transferred through a gas‐permeable membrane into the flow system. The membrane enabled the separation of the electrolyte from the biocatalysts in the flow system, where H2 and O2 served as electron mediators for the biocatalysts. We demonstrate the production of methylated N‐heterocycles from diamines with up to 99 % product formation as well as excellent regioselective labeling with stable isotopes. Our platform can be applied for a broad panel of oxidoreductases to exploit electrical energy for the synthesis of fine chemicals.
Author(s)
Al-Shameri, Ammar
Technische Universität Berlin
Petrich, Marie-Christine
Technische Universität Berlin
junge Puring, Kai
Fraunhofer-Institut für Umwelt-, Sicherheits- und Energietechnik UMSICHT
Apfel, Ulf-Peter
Fraunhofer-Institut für Umwelt-, Sicherheits- und Energietechnik UMSICHT
Nestl, Bettina M.
Universität Stuttgart
Lauterbach, Lars
Technische Universität Berlin
Zeitschrift
Angewandte Chemie. International edition
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DOI
10.1002/anie.202001302
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Language
Englisch
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UMSICHT Oh
Tags
  • electrochemical bioca...

  • hydrogenases

  • imine reductases

  • isotopic labeling

  • N-heterocycles

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