Photopatternable, Branched Polymer Hydrogels Based on Linear Macromonomers for 3D Cell Culture Applications
Abstract
Photochemical ligation strategies in hydrogel materials are crucial to model spatiotemporal phenomena that occur in the natural extracellular matrix. We here describe the use of cyclic 1,2-dithiolanes to cross-link with norbornene on linear poly(ethylene glycol) polymers through UV irradiation in a rapid and byproduct-free manner, resulting in branched macromolecular architectures and hydrogel materials from low-viscosity precursor solutions. Oscillatory rheology and NMR data indicate the one-pot formation of thioether and disulfide cross-links. Spatial and temporal control of the hydrogel mechanical properties and functionality was demonstrated by oscillatory rheology and confocal microscopy. A cytocompatible response of NIH 3T3 fibroblasts was observed within these materials, providing a foothold for further exploration of this photoactive cross-linking moiety in the biomedical field.
Author(s)
Tong, Ciqing
Department of Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, RA Leiden, The Netherlands
Wondergem, Joeri A.J.
Huygens-Kamerlingh Onnes Laboratory, Leiden Institute of Physics, Leiden University, RA Leiden, The Netherlands
Kieltyka, Roxanne E.
Department of Supramolecular and Biomaterials Chemistry, Leiden Institute of Chemistry, RA Leiden, The Netherlands
