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  4. Low-energy electron irradiation efficiently inactivates the gram-negative pathogen rodentibacter pneumotropicus
 
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2020
Journal Article
Titel

Low-energy electron irradiation efficiently inactivates the gram-negative pathogen rodentibacter pneumotropicus

Titel Supplements
A new method for the generation of bacterial vaccines with increased efficacy
Abstract
Bacterial pathogens cause severe infections worldwide in livestock and in humans, and antibiotic resistance further increases the importance of prophylactic vaccines. Inactivated bacterial vaccines (bacterins) are usually produced via incubation of the pathogen with chemicals such as formaldehyde, which is time consuming and may cause loss of immunogenicity due to the modification of structural components. We evaluated low-energy electron irradiation (LEEI) as an alternative method to generate a bacterin. Rodentibacter pneumotropicus, an invasive Gram-negative murine pathogen, was inactivated with LEEI and formaldehyde. LEEI resulted in high antigen conservation, and LPS activity was significantly better maintained when compared with formaldehyde treatment. Immunization of mice with LEEI-inactivated R. pneumotropicus elicited a strong immune response with no detectable bacterial burden upon sublethal challenge. The results of this study suggest the inactivation of bacteria with LEEI as an alternative, fast and efficient method to generate bacterial vaccines with increased efficacy.
Author(s)
Fertey, Jasmin orcid-logo
Fraunhofer-Institut für Zelltherapie und Immunologie IZI
Bayer, Lea
Fraunhofer-Institut für Zelltherapie und Immunologie IZI
Kähl, Sophie
Universität Leipzig
Haji, Rukiya M.
Fraunhofer-Institut für Zelltherapie und Immunologie IZI
Burger-Kentischer, Anke
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB
Thoma, Martin
Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA
Standfest, Bastian
Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA
Baums, Christoph Georg
Universität Leipzig
Casado, Javier Portillo
Fraunhofer-Institut für Organische Elektronik, Elektronenstrahl- und Plasmatechnik FEP
Rögner, Frank-Holm
Fraunhofer-Institut für Organische Elektronik, Elektronenstrahl- und Plasmatechnik FEP
Schönfelder, Jessi
Fraunhofer-Institut für Organische Elektronik, Elektronenstrahl- und Plasmatechnik FEP
Grunwald, Thomas
Fraunhofer-Institut für Zelltherapie und Immunologie IZI
Ulbert, Sebastian
Fraunhofer-Institut für Zelltherapie und Immunologie IZI
Zeitschrift
Vaccines
Thumbnail Image
DOI
10.3390/vaccines8010113
Language
Englisch
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Fraunhofer-Institut für Zelltherapie und Immunologie IZI
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB
Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA
Fraunhofer-Institut für Organische Elektronik, Elektronenstrahl- und Plasmatechnik FEP
Tags
  • Bacterin

  • Electron beam

  • Inactivation of bacte...

  • Low-energy electron i...

  • Rodentibacter

  • Bakterien

  • Elektronenstrahl

  • Strahlentherapie

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