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  4. Comparative characterization of two galectins excreted-secreted from intestine-dwelling parasitic versus free-living females of the soil-transmitted nematode Strongyloides
 
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2018
Journal Article
Titel

Comparative characterization of two galectins excreted-secreted from intestine-dwelling parasitic versus free-living females of the soil-transmitted nematode Strongyloides

Abstract
Helminths are complex pathogens that ensure their long-term survival by influencing the immune responses of their host. Excretory/secretory products (ESP) can exert immunoregulatory effects which foster parasite survival. Galectins represent a widespread group of v-galactoside-binding proteins which are involved in a multitude of biological processes operative in parasite-host interaction. We had earlier identified seven galectins in Strongyloides ratti, four of them detected in the ESP of distinct developmental stages of the parasite. In the present report, we focused on the characterization of two of them, Sr-galectin-1 (Sr-Gal-1) and Sr-galectin-3 (Sr-Gal-3). While Sr-Gal-3 expression was strongest in parasitic females, Sr-Gal-1 was predominantly expressed in free-living females. Both proteins were cloned and recombinantly expressed in an E. coli expression system. Their glycan-binding activity was verified by haemagglutination and glycan array analysis. Furthermore, primary immunological activities of the Sr-galectins were initially investigated by the application of an in vitro mucosal 3D-culture model, comprising of mucosa-associated epithelial and dendritic cells. The Sr-galectins stimulated preferentially the release of the type 2 cytokines thymic stromal lymphopoietin and IL-22, a first indication for immunoregulatory activity. In addition, the Sr-galectins dose-dependently fostered cell migration. Our results confirm the importance of these carbohydrate-binding proteins in host-parasite-interaction by indicating possible interaction with the host mucosa-associated cells.
Author(s)
Ditgen, Dina
Anandarajah, Emmanuela M.
Reinhardt, Anika
Younis, Abuelhassan Elshazly
Witt, Susanne
Hansmann, Jan
Fraunhofer-Institut für Silicatforschung ISC
Lorenz, Eva
García-Hernández, Marisela
Paclik, Daniela
Soblik, Hanns
Jolodar, Abbas
Seeberger, Peter H.
Liebau, Eva
Brattig, Norbert W.
Zeitschrift
Molecular and Biochemical Parasitology
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DOI
10.1016/j.molbiopara.2018.08.008
Language
English
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Fraunhofer-Institut für Silicatforschung ISC
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