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  4. Rapid analysis of cell-nanoparticle interactions using single-cell raman trapping microscopy
 
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2018
Journal Article
Titel

Rapid analysis of cell-nanoparticle interactions using single-cell raman trapping microscopy

Abstract
Iron oxide nanoparticles have been used in preclinical studies to label stem cells for non‐invasive tracking and homing. The search continues for novel particle candidates that are suitable for clinical applications. Since standard analyses to investigate cell-particle interactions and safety are labor‐intensive, an efficient procedure is required to guide future particle development and to exclude adverse health effects. The application of combined Raman trapping microscopy with fluidic chips is reported for the analysis of single cells labeled with different types of aminated iron oxide particles. Multivariate data analysis revealed Raman signal differences that could be clearly assigned to cell-particle interactions and cytotoxicity, respectively. A validation dataset verified that more than 95 % of the spectra were correctly classified. Thus, our approach enables rapid discrimination of non‐hazardous from cytotoxic nanoparticles as a prerequisite for safe clinical applications.
Author(s)
Steinke, Maria
Fraunhofer-Institut für Silicatforschung ISC
Zunhammer, Florian
Chatzopoulou, Elisavet I.
Teller, Henrik
Schütze, Karin
Walles, Heike
Fraunhofer-Institut für Silicatforschung ISC
Rädler, Joachim O.
Grüttner, Cordula
Zeitschrift
Angewandte Chemie. International edition
Thumbnail Image
DOI
10.1002/anie.201713151
Language
English
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Fraunhofer-Institut für Silicatforschung ISC
Tags
  • Zelle

  • Nanopartikel

  • Stammzelle

  • Raman-Mikroskopie

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