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  4. A multilayered electrospun graft as vascular access for hemodialysis
 
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2017
Journal Article
Title

A multilayered electrospun graft as vascular access for hemodialysis

Abstract
Despite medical achievements, the number of patients with end-stage kidney disease keeps steadily raising, thereby entailing a high number of surgical and interventional procedures to establish and maintain arteriovenous vascular access for hemodialysis. Due to vascular disease, aneurysms or infection, the preferred access-an autogenous arteriovenous fistula-is not always available and appropriate. Moreover, when replacing small diameter blood vessels, synthetic vascular grafts possess well-known disadvantages. A continuous multilayered gradient electrospinning was used to produce vascular grafts made of collagen type I nanofibers on luminal and adventitial graft side, and poly-ɛ-caprolactone as medial layer. Therefore, a custom-made electrospinner with robust environmental control was developed. The morphology of electrospun grafts was characterized by scanning electron microscopy and measurement of mechanical properties. Human microvascular endothelial cells were cultured in the graft under static culture conditions and compared to cultures obtained from dynamic continuous flow bioreactors. Immunofluorescent analysis showed that endothelial cells form a continuous luminal layer and functional characteristics were confirmed by uptake of acetylated low-density-lipoprotein. Incorporation of vancomycin and gentamicin to the medial graft layer allowed antimicrobial inhibition without exhibiting an adverse impact on cell viability. Most striking a physiological hemocompatibility was achieved for the multilayered grafts.
Author(s)
Radakovic, Dejan
Reboredo, Jenny W.
Helm, M.
Fraunhofer-Institut für Silicatforschung ISC  
Weigel, Tobias  
Schürlein, Sebastian
Kupczyk, E.
Leyh, Rainer G.
Walles, Heike
Fraunhofer-Institut für Silicatforschung ISC  
Hansmann, Jan  
Fraunhofer-Institut für Silicatforschung ISC  
Journal
PLoS one. Online journal  
Open Access
DOI
10.1371/journal.pone.0185916
Link
Link
Language
English
Fraunhofer-Institut für Silicatforschung ISC  
Keyword(s)
  • Hämodialyse

  • Transplantation

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