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2017
Journal Article
Title
Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
Abstract
The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.
Author(s)
File(s)
Rights
CC BY 4.0: Creative Commons Attribution
Language
English