Candida guilliermondii as a potential biocatalyst for the production of long-chain a,o-dicarboxylic acids

Objectives: To explore Candida guilliermondii for the production of long-chain dicarboxylic acids (DCA), we performed metabolic pathway engineering aiming to prevent DCA consumption during v-oxidation, but also to increase its production via the o-oxidation pathway. Results: We identified the major v- and o-oxidation pathway genes in C. guilliermondii and performed first steps in the strain improvement. A double pox disruption mutant was created that slowed growth with oleic acid but showed accelerated DCA degradation. Increase in DCA production was achieved by homologous overexpression of a plasmid borne cytochrome P450 monooxygenase gene. Conclusion: C. guilliermondii is a promising biocatalyst for DCA production but further insight into its fatty acid metabolism is necessary.