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  4. Human barrier models for the in vitro assessment of drug delivery
 
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2017
Journal Article
Titel

Human barrier models for the in vitro assessment of drug delivery

Abstract
In vitro test systems gain increasing importance in preclinical studies to increase the predictivity and reduce animal testing. Of special interest herein are barrier tissues that guard into the human body. These barriers are formed by highly specialized tissues such as the skin, the airways, and the intestine. However, to recapitulate these tissues, researchers are currently restricted by a lack of suitable supporting scaffolds. In this study, we present biological scaffolds based on decellularized porcine gut segments that offer a natural environment for cell growth and differentiation. Employing these scaffolds, human barrier models of the skin, the airways, and the intestine that mimic the natural histological architecture of the respective tissue are generated. These models show tissue specific barrier properties, such as the stratification of the skin, the mucociliary phenotype of the airways, and polarization of the intestinal epithelium. To investigate the transport characteristics of the intestinal test system, we incubated the tissue models with fluorescein (Papp <1 x 106 cm/s), propranolol (Papp >7 x 106 cm/s), and rhodamin123 (ratio 2.45). The here presented biological scaffolds facilitate the in vitro generation of human barrier models that might represent useful tools for drug delivery studies.
Author(s)
Schweinlin, Matthias
Rossi, Angela
Lodes, Nina
Lotz, Christian
Hackenberg, Stephan
Steinke, Maria
Walles, Heike
Groeber-Becker, Florian
Zeitschrift
Drug Delivery and Translational Research
Thumbnail Image
DOI
10.1007/s13346-016-0316-9
Language
English
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Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB
Fraunhofer-Institut für Silicatforschung ISC
Tags
  • alternative to animal...

  • drug delivery

  • human barrier tissue

  • In vitro model

  • tissue engineering

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