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  4. Additive manufacturing of collagen scaffolds by three-dimensional plotting of highly viscous dispersions
 
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2016
Journal Article
Titel

Additive manufacturing of collagen scaffolds by three-dimensional plotting of highly viscous dispersions

Abstract
Additive manufacturing (AM) allows the free form fabrication of three-dimensional (3D) structures with distinct external geometry, fitting into a patient-specific defect, and defined internal pore architecture. However, fabrication of predesigned collagen scaffolds using AM-based technologies is challenging due to the low viscosity of collagen solutions, gels or dispersions commonly used for scaffold preparation. In the present study, we have developed a straightforward method which is based on 3D plotting of a highly viscous, high density collagen dispersion. The swollen state of the collagen fibrils at pH4 enabled the homogenous extrusion of the material, the deposition of uniform strands and finally the construction of 3D scaffolds. Stabilization of the plotted structures was achieved by freeze-drying and chemical crosslinking with the carbodiimide EDC. The scaffolds exhibited high shape and dimensional fidelity and a hierarchical porosity consisting of macropores generated by strand deposition as well as an interconnected microporosity within the strands as result of the freeze-drying process. Cultivation of human mesenchymal stromal cells on the scaffolds, with and without adipogenic or osteogenic stimulation, revealed their cytocompatibility and potential applicability for adipose and bone tissue engineering.
Author(s)
Lode, Anja
TU Dresden
Meyer, Michael
FILK, Freiberg
Brüggemeier, Sophie
TU Dresden
Paul, Birgit
TU Dresden
Baltzer, Hagen
FILK, Freiberg
Schröpfer, Michaela
FILK, Freiberg
Winkelmann, Claudia
Fraunhofer-Institut für Werkstoff- und Strahltechnik IWS
Sonntag, Frank
Fraunhofer-Institut für Werkstoff- und Strahltechnik IWS
Gelinsky, Michael
TU Dresden
Zeitschrift
Biofabrication
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DOI
10.1088/1758-5090/8/1/015015
Language
English
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Fraunhofer-Institut für Werkstoff- und Strahltechnik IWS
Tags
  • additive manufacturin...

  • Rapid Prototyping

  • extrusion

  • Collagen

  • freeze-drying

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