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  4. Identification of two-pore domain potassium channels as potent modulators of osmotic volume regulation in human T lymphocytes
 
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2013
Journal Article
Title

Identification of two-pore domain potassium channels as potent modulators of osmotic volume regulation in human T lymphocytes

Abstract
Many functions of T lymphocytes are closely related to cell volume homeostasis and regulation, which utilize a complex network of membrane channels for anions and cations. Among the various potassium channels, the voltage-gated K(v)1.3 is well known to contribute greatly to the osmoregulation and particularly to the potassium release during the regulatory volume decrease (RVD) of T cells faced with hypotonic environment. Here we address a putative role of the newly identified two-pore domain (K-2P) channels in the RVD of human CD4(+) T lymphocytes, using a series of potent well known channel blockers. In the present study, the pharmacological profiles of RVD inhibition revealed K(2P)5.1 and K(2P)18.1 as the most important K-2P channels involved in the RVD of both naive and stimulated T cells. The impact of chemical inhibition of K(2P)5.1 and K(2P)18.1 on the RVD was comparable to that of K(v)1.3. K(2P)9.1 also notably contributed to the RVD of T cells but the extent of this contribution and its dependence on the activation status could not be unambiguously resolved. In summary, our data provide first evidence that the RVD-related potassium efflux from human T lymphocytes relies on K-2P channels.
Author(s)
Andronic, J.
Bobak, N.
Bittner, S.
Ehling, P.
Kleinschnitz, C.
Herrmann, A.M.
Zimmermann, H.  
Sauer, M.
Wiendl, H.
Budde, T.
Meuth, S.G.
Sukhorukov, V.L.
Journal
Biochimica et Biophysica Acta. Biomembranes  
Funder
Deutsche Forschungsgemeinschaft DFG  
DOI
10.1016/j.bbamem.2012.09.028
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Fraunhofer-Institut für Biomedizinische Technik IBMT  
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