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  4. Functionalization of PDMS modified and plasma activated two-component polyurethane coatings by surface attachment of enzymes
 
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2013
Journal Article
Title

Functionalization of PDMS modified and plasma activated two-component polyurethane coatings by surface attachment of enzymes

Abstract
This article describes a new strategy for coupling the enzyme horseradish peroxidase to a two-component polyurethane (2C-PUR) coating. A stable polymer conjugate was achieved by combining the enzyme and the 2C-PUR coating which was modified with poly(dimethylsiloxane) (PDMS), located at the surface. An atmospheric pressure plasma jet system was used to convert alkyl groups from the PDMS into polar silanol functionalities. This conversion was proven by X-ray photoelectron spectroscopy and dynamic contact angle measurements. In addition, the stability of the activated 2C-PUR surface containing silanol groups was determined by measuring the contact angle as a function of time. Compared to the non-modified 2C-PUR systems the one with PDMS displayed a higher stability over a time period over 28 h. In a silanization process the coating was treated with (3-aminopropyl) trimethoxysilane and the enzyme was subsequently immobilized to the coating via the cross linker glutaraldehyde to receive new biomimetic catalytic/enzymatic functions. The chemical immobilization (chemisorption) of the enzyme to the surface showed statistically significant higher biological activity as compared to references samples without using a cross linker (physisorption). The presented technique offers the opportunity to design new and smart multifunctional surface coatings which employ biomimetic capabilities.
Author(s)
Kreider, A.
Fraunhofer-Institut für Fertigungstechnik und Angewandte Materialforschung IFAM  
Richter, K.
Fraunhofer-Institut für Fertigungstechnik und Angewandte Materialforschung IFAM  
Sell, S.
Fraunhofer-Institut für Fertigungstechnik und Angewandte Materialforschung IFAM  
Fenske, M.
Fraunhofer-Institut für Fertigungstechnik und Angewandte Materialforschung IFAM  
Tornow, C.
Fraunhofer-Institut für Fertigungstechnik und Angewandte Materialforschung IFAM  
Stenzel, V.
Fraunhofer-Institut für Fertigungstechnik und Angewandte Materialforschung IFAM  
Grunwald, I.
Fraunhofer-Institut für Fertigungstechnik und Angewandte Materialforschung IFAM  
Journal
Applied surface science  
Project(s)
AFP-Lack II
Funder
Bundesministerium für Bildung und Forschung BMBF (Deutschland)  
DOI
10.1016/j.apsusc.2013.02.080
Language
English
Fraunhofer-Institut für Fertigungstechnik und Angewandte Materialforschung IFAM  
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