Natural frost protection coupling antifreeze proteins to a pu coating inhibits ice deposition

The removal of ice build-up generally involves the application of both energy and environmentally-unfriendly products. Antifreeze proteins (AFP) are produced by certain fish, insects and bacteria in order to survive under conditions below the freezing point. Antifreeze proteins differ radically in their primary, secondary, and tertiary structures. The AFPs of fish also vary in structure and are subdivided into different types. XPS spectra were recorded in order to draw conclusions about the excess hydroxyl groups in the 2K polyurethane system. In addition to the spectroscopic methods for surface analysis mentioned above, fluorescence microscopy was also used to detect the coupling of BSA-FITC. This method allowed the successful coupling of the model protein to be demonstrated. The amino groups of the silane were used to subsequently couple biomolecules to the surface. In order to follow the immobilization reaction using fluorescence microscopy, BSA-FITC was used as a mo del protein.