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  4. Direct detection of tuberculosis infection in blood serum using three optical label-free approaches
 
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2008
Journal Article
Title

Direct detection of tuberculosis infection in blood serum using three optical label-free approaches

Abstract
We describe a new and rapid method for diagnosing tuberculosis-specific antibodies in undiluted human blood serum by surface sensitive biosensors. An integrated optical grating coupler, an interferometric biosensor and a device based on the reflectometric interference spectroscopy (BIAffinity, Analytik Jena AG) were used to determine tuberculosis-specific antibodies, which are present in blood serum of infected persons. The specific sensor surface was generated by immobilized 38-kDa tuberculosis antigen and real-time detection was performed without any label in all cases. Unspecific binding effects caused by serum proteins were suppressed by appropriate coupling chemistry. 1,4-Phenylenediisothiocyanate was used as a crosslinker for the coupling of the antigen achieving the effect of complete surface coverage. This enables label-free serodiagnosis of tuberculosis infection with a specificity of 100% and a sensitivity of 75%. Based on our results, we anticipate that a label-free detection procedure with one or more marker antigens is possible for a rapid, economical and accurate routine diagnosis of tuberculosis and other infectious diseases in the future.
Author(s)
Nagel, T.
Ehrentreich-Förster, E.
Singh, M.
Schmitt, K.
Fraunhofer-Institut für Physikalische Messtechnik IPM  
Brandenburg, Albrecht  
Fraunhofer-Institut für Physikalische Messtechnik IPM  
Berka, A.
Bier, F.
Journal
Sensors and Actuators. B  
DOI
10.1016/j.snb.2007.10.009
Language
English
Fraunhofer-Institut für Biomedizinische Technik IBMT  
Fraunhofer-Institut für Physikalische Messtechnik IPM  
Keyword(s)
  • Mycobacterium tuberculosis

  • label-free

  • blood serum

  • grating coupler

  • interferometric biosensor

  • RIfS

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