Nanobiosensors: A powerful Technology for Early Detection of Plant Parasitic Nematodes

Nanotechnology emerged as a critical force in agriculture and the most vital technology. Nanobiotechnology offers significant benefits for farmers to control their output by regulating nutrient use via nano-fertilizers, rationalizing agrochemical use, detecting, and treating disease–host interactions, molecular interactions with nanocarriers (nano biosensors) for plant disease diagnosis, and removing contaminants from water and soil, farm management, and current trends include salinity soil treatment. Nanosensors can also detect pathogens, moisture, fertilizers, and acidity of soil to improve products used to protect the plant, reduce nutrient leakage, and increase yield production due to effective management of nutrients. This section will look at bio-nanosensors based on soil sensors and nano-sensors that can be conjugated with nematode biomarkers to deliver the easy and quick detection of nematode plant diseases. They are also integrating biomarker with nanosensor to improve the vision and application of biosensor technology. According to the Docking test, Selectivity and specificity of the protein (MiPFN3) found specifically in root–knot nematodes and is not present in plants or other organisms. Therefore, inhibiting this protein would be a highly specific and targeted approach for controlling these nematodes, minimizing potential harm to non-target organisms. by generating a random pool of sequences using the shuffle-seq tool. In-silico Aptamer that can interact and inhibit MiPFN3 was designed. Graphical Abstract: Schematic representation to illustrate Plant parasitic nematode diagnostics with nano biosensors as a novel extremely sensitive tool in sustainable agriculture.