Stationary fluidics: Moving target molecules on beads through non-moving liquids for molecular diagnostic assays

Commonly, microfluidic devices are constructed to move fluidics. For molecular diagnostics assays which include steps like PCR, this practically always involves a more or less complicated set of external pumps, valves and liquid controls. In the presented experiment, the fluid after sample introduction remains stationary and the main bioactive sample molecules are moved through a chain of reaction compartments which contain different reagents. The big advantage of this concept is the lack of any external fluid actuation/control. Results on sample carry-over experiments and complete assays will be given.