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  4. Smart Slides for Optical Monitoring of Cellular Processes
 
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2024
Journal Article
Title

Smart Slides for Optical Monitoring of Cellular Processes

Abstract
The molecules released by cells are a fingerprint of their current state. Methods that measure them with high spatial and temporal resolution may provide valuable insights into cell physiology and diseases. Here, a nanosensor coating is developed that transforms standard cell culture materials/dishes into "Smart Slides" capable of optically monitoring biochemical efflux from cells. For this purpose, single wall carbon nanotubes (SWCNTs) that are fluorescent in the beneficial near-infrared (NIR, 850-1700 nm) window are used. They are chemically tailored to detect the neurotransmitter dopamine by a change in fluorescence intensity. These nanosensors are spin-coated on glass substrates and it is shown that such sensor layers can be sterilized by UV light and can be stored in dry condition or buffer for at least 6 weeks and have little influence on cell viability. The optimal sensor density to maximize sensitivity is also identified. Finally, these substrates are used to image dopamine release from neuronal cells cultivated on top in the presence of various psychotropic substances, which represents a system to test pharmaceuticals for neurological or neurodegenerative diseases. Therefore, Smart Slides are a powerful tool to monitor cellular processes in cell culture systems.
Author(s)
Ackermann, Julia
Fraunhofer-Institut für Mikroelektronische Schaltungen und Systeme IMS  
Reger, Eline
Fraunhofer-Institut für Mikroelektronische Schaltungen und Systeme IMS  
Jung, Sebastian
Ruhr-Universität Bochum  
Mohr, Jennifer
Ruhr-Universität Bochum  
Herbertz, Svenja  
Fraunhofer-Institut für Mikroelektronische Schaltungen und Systeme IMS  
Seidl, Karsten  
Fraunhofer-Institut für Mikroelektronische Schaltungen und Systeme IMS  
Kruss, Sebastian  
Fraunhofer-Institut für Mikroelektronische Schaltungen und Systeme IMS  
Journal
Advanced Functional Materials  
Project(s)
EXC 2033: Ruhr Explores Solvation  
Center for Solvation Science  
Funder
Deutsche Forschungsgemeinschaft -DFG-, Bonn  
Bundesministerium für Bildung und Forschung -BMBF-  
Open Access
DOI
10.1002/adfm.202309064
10.24406/publica-2106
File(s)
Adv Funct Materials - 2023 - Ackermann - Smart Slides for Optical Monitoring of Cellular Processes.pdf (1.85 MB)
Rights
CC BY 4.0: Creative Commons Attribution
Language
English
Fraunhofer-Institut für Mikroelektronische Schaltungen und Systeme IMS  
Keyword(s)
  • near-infrared (NIR) fluorescence

  • imaging

  • biosensors

  • carbon nanotubes

  • dopamine

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