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2022
Conference Paper
Titel
Surface optimization of dental implants with laser surface texturing and silver coating
Abstract
The risk of bacterial inflammation at the interface between implant and tissue exists following the implantation of a dental prosthesis. Nearly half of implants are at risk of colonisation by pathogenic bacteria, which is associated with the occurrence of peri-implant mucositis. This disease can develop into peri-implantitis and thereby trigger a severe inflammatory process. The occurrence of peri-implantitis includes different phases. The initial attachment of microorganisms is only possible by pioneer bacteria, such as the gram-positive streptococci. Since the pathogenic cannot form a biofilm unless attached to a surface, the attachment of the pioneer bacteria is crucial for the onset of peri-implantitis. Due to the flexibility and contact free process, laser material processing is used for the surface structuring of several materials. In the biomedical field, laser-based surface texturing enables the production of implants with improved biological reaction surfaces to positively influence protein adsorption and cell adhesion. This paper presents laser texturing and silver coating to reduce initial biofilm formation on Ti6Al4V. The laser processing includes the manufacturing of LIPSS (Laser Induced Periodic Surface Structures), which enables the functionalisation of the surface. Furthermore, the surfaces are coated with silver to act as an inhibitor of biofilm formation. The implant material undergoes an in vitro culture of the microorganism Streptococcus salivarius in order to determine the biofilm formation applying both techniques. The analysis was realized by fluorescence microscopy with the application of 4', 6-diamidino-2-phenylindole (DAPI) on the adhered biofilm. Results show that the surface modification plays a major role in the inhibition of biofilm formation.
Author(s)
Souza Schweitzer, Luiz Guilherme De