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  4. Identification of two early blood biomarkers ACHE and CLEC12A for improved risk stratification of critically ill COVID-19 patients
 
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2023
Journal Article
Title

Identification of two early blood biomarkers ACHE and CLEC12A for improved risk stratification of critically ill COVID-19 patients

Abstract
In order to identify biomarkers for earlier prediction of COVID-19 outcome, we collected blood samples from patients with fatal outcomes (non-survivors) and with positive clinical outcomes (survivors) at ICU admission and after seven days. COVID-19 survivors and non-survivors showed significantly different transcript levels for 93 genes in whole blood already at ICU admission as revealed by RNA-Seq. These differences became even more pronounced at day 7, resulting in 290 differentially expressed genes. Many identified genes play a role in the differentiation of hematopoietic cells. For validation, we designed an RT-qPCR assay for C-type lectin domain family 12 member A (CLEC12A) and acetylcholinesterase (ACHE), two transcripts that showed highest potential to discriminate between survivors and non-survivors at both time points. Using our combined RT-qPCR assay we examined 33 samples to accurately predict patient survival with an AUROC curve of 0.931 (95% CI = 0.814–1.000) already at ICU admission. CLEC12A and ACHE showed improved prediction of patient outcomes compared to standard clinical biomarkers including CRP and PCT in combination (AUROC = 0.403, 95% CI = 0.108–0.697) or SOFA score (AUROC = 0.701 95% CI = 0.451–0.951) at day 0. Therefore, analyzing CLEC12A and ACHE gene expression from blood may provide a promising approach for early risk stratification of severely ill COVID-19 patients.
Author(s)
Kattner, Simone
Müller, Jan  
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
Glanz, Karolina  
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
Manoochehri, Mehdi
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
Sylvester, Caroline Hannelore
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
Vainshtein, Yevhen  orcid-logo
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
Berger, Marc Moritz
N
Brenner, Thorsten
Sohn, Kai  
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
Journal
Scientific Reports  
Open Access
DOI
10.1038/s41598-023-30158-1
Language
English
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
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