• English
  • Deutsch
  • Log In
    Password Login
    Research Outputs
    Fundings & Projects
    Researchers
    Institutes
    Statistics
Repository logo
Fraunhofer-Gesellschaft
  1. Home
  2. Fraunhofer-Gesellschaft
  3. Artikel
  4. Quantification of substitution of gelatin methacryloyl. Best practice and current pitfalls
 
  • Details
  • Full
Options
2018
Journal Article
Title

Quantification of substitution of gelatin methacryloyl. Best practice and current pitfalls

Abstract
Cross-linkable gelatin methacryloyl (GM) is widely used for the generation of artificial extracellular matrix (ECM) in tissue engineering. However, the quantification of modified groups in GM is still an unsolved issue, although this is the key factor for tailoring the physicochemical material properties. In this contribution, 1H-13C-HSQC NMR spectra are used to gain detailed structural information on GMs and of 2-fold modified gelatin containing methacryloyl and acetyl groups (GMAs). Distinctive identification of methacrylate, methacrylamide, and acetyl groups present in GMs and GMAs revealed an overlap of methacrylamide and modified hydroxyproline signals in the 1H NMR spectrum. Considering this, we suggest a method to quantify methacrylate and methacrylamide groups in GMs precisely based on simple 1H NMR spectroscopy with an internal standard. Quantification of acetylation in GMAs is also possible, yet, 2D NMR spectra are necessary. The described methods allow direct quantification of modified groups in gelatin derivatives, making them superior to other, indirect methods known so far.
Author(s)
Claaßen, Christiane  
Claaßen, Marc H.
Truffault, Vincent
Sewald, Lisa  
Tovar, Günter E.M.  
Borchers, Kirsten  
Southan, Alexander  
Journal
Biomacromolecules  
DOI
10.1021/acs.biomac.7b01221
Language
English
Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB  
  • Cookie settings
  • Imprint
  • Privacy policy
  • Api
  • Contact
© 2024