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  4. Osmotic stress interferes with DNA damage response and H2AX phosphorylation in human keratinocytes
 
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2022
Journal Article
Title

Osmotic stress interferes with DNA damage response and H2AX phosphorylation in human keratinocytes

Abstract
The human skin and in particular its outermost layer, the epidermis, protects the body from potentially harmful substances, radiation as well as excessive water loss. However, the interference between the various stress responses of the epidermal keratinocytes, which often occur simultaneously, is largely unknown. The focus of this study was to investigate the interference between osmotic stress and DNA damage response. In addition to revealing the already well-described regulation of diverse gene sets, for example, cellular processes such as transcription, translation, and metabolic pathways (e.g., the KEGG citrate cycle and Reactome G2/M checkpoints), gene expression analysis of osmotically stressed keratinocytes revealed an influence on the transcription of genes also related to UV-induced DNA damage response. A gene network regulating the H2AX phosphorylation was identified to be regulated by osmotic stress. To analyze and test the interference between osmotic stress and DNA damage response, which can be triggered by UV stress on the one hand and oxidative stress on the other, in more detail, primary human keratinocytes were cultured under osmotic stress conditions and subsequently exposed to UV light and H2O2, respectively. γH2AX measurements revealed lower γH2AX levels in cells previously cultured under osmotic stress conditions.
Author(s)
Hoen, Laura
Hochschule für angewandte Wissenschaften Coburg
Rudisch, Christoph
Hochschule für angewandte Wissenschaften Coburg
Wick, Michael
Hochschule für angewandte Wissenschaften Coburg
Indenbirken, Daniela
Leibniz-Institut für Experimentelle Virologie HPI Hamburg
Grundhoff, Adam
Leibniz-Institut für Experimentelle Virologie HPI Hamburg
Wegwitz, Florian
Universitätsmedizin Göttingen UMG
Kalkhof, Stefan  orcid-logo
Fraunhofer-Institut für Zelltherapie und Immunologie IZI  
Hildebrand, Janosch
Hochschule für angewandte Wissenschaften Coburg
Journal
Cells  
Open Access
DOI
10.3390/cells11060959
Additional full text version
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Language
English
Fraunhofer-Institut für Zelltherapie und Immunologie IZI  
Keyword(s)
  • Keratinocytes

  • UV radiation

  • Gene regulation

  • DNA repair disorders

  • Osmotic stress

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