Isolation and identification of Cyclin E1 isoforms in murine hepatocellular carcinomas (HCC)

Cyclin E1 is involved in controlling G1/S-phase progression during the cell cycle. Earlier results indicated that Cyclin E1 misregulation correlates with tumor progression in some types of human cancer. Short isoforms of Cyclin E1 mRNA were found in human tissues. In the present studies we investigated if short isoforms of Cyclin E1 might be linked to murine hepatocarcinogenesis. We first cloned and sequenced the yet unknown distal part f the genomic mouse Cyclin E1. Sequence comparison of the mouse and human Cyclin E1 locus revealed a strong conservation of the iatron-exon structure, whereas only little homology was found on the DNA level.