Device and method for manipulating biological cells and method of manufacturing the device

The invention relates to a device (10) for manipulating biological cells, the device (10) comprising: at least one container (22) for cultivating biological cells, the container (22) having an interior space (11), and at least one electrode (14) for manipulating the biological cells, wherein the device (10) comprises at least one separator layer (20), the at least one separator layer (20) being arranged at least between the at least one electrode (14) and the interior space (11) such that the at least one electrode (14) is arranged outside of the interior space (11). The invention further relates to a method (300) for manipulating biological cells. The invention provides a device (10) and a method (300) for manipulating biological cells that avoids the degradation of the cells in containers (22) during the manipulation of the cells with electric fields.