• English
  • Deutsch
  • Log In
    Password Login
    Research Outputs
    Fundings & Projects
    Researchers
    Institutes
    Statistics
Repository logo
Fraunhofer-Gesellschaft
  1. Home
  2. Fraunhofer-Gesellschaft
  3. Artikel
  4. Suppression of growth factor-mediated MAP kinase activation by v-raf in macrophages, a putative role for the MKP-1 phosphatase
 
  • Details
  • Full
Options
1995
Journal Article
Title

Suppression of growth factor-mediated MAP kinase activation by v-raf in macrophages, a putative role for the MKP-1 phosphatase

Abstract
Many tyrosine kinase growth factor receptors activate the MAP Kinase (MAPK) pathway by stimulating the activity of the RAF kinase. In some, but not all cell types, the expression of activated RAF is sufficient to induce constitutive MAPK activation. In BAC-1.2F5 macrophages the expression of virally activated RAF does not correlate with constitutive MAPK activation; on the contrary, growth factor-mediated stimulation of MAPK activity is suppressed in these cells. Suppression correlates with v-RAF expression, as MAPK activation is normal in a revertant cell line that stopped expressing v-RAF. Inhibition of MAPK activation is associated with lack of ERK-2 tyrosine phosphorylation, and is not due to the suppression of CSF-1-mediated MEK activation. Pretreatment with vanadate restores growth factor-stimulated activation and tyrosine phosphorylation of MAPK in v-RAF-expressing macrophages, indicating the involvement of a tyrosine phosphatase.
Author(s)
Krautwald, S.
Dent, P.
Ruthenberg, K.
Büscher, D.
Baccarini, M.
Journal
Oncogene  
Language
English
ITA  
Keyword(s)
  • amino acid

  • cancer

  • cell line

  • colony stimulating factor

  • enzyme activation

  • gene expression regulation

  • genetic aspect

  • growth factor

  • immunology

  • macrophage

  • molecular biology

  • phosphorylation

  • protein kinase

  • tyrosine

  • Cookie settings
  • Imprint
  • Privacy policy
  • Api
  • Contact
© 2024