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Utilising the left-helical conformation of L-DNA for analysing different marker types on a single universal microarray platform

: Hauser, N.C.; Martinez, R.; Jacob, A.; Rupp, S.; Hoheisel, J.D.; Matysiak, S.

Postprint (PDF; )

Nucleic Acids Research 34 (2006), No.18, pp.5101-5111
ISSN: 0305-1048
ISSN: 0301-5610
ISSN: 1362-4962
Journal Article, Electronic Publication
Fraunhofer IGB ()

L-DNA is the perfect mirror-image form of the naturally occurring d-conformation of DNA. Therefore, L-DNA duplexes have the same physical characteristics in terms of solubility, duplex stability and selectivity as D-DNA but form a left-helical double-helix. Because of its chiral difference, L-DNA does not bind to its naturally occurring D-DNA counterpart, however. We analysed some of the properties that are typical for L-DNA. For all the differences, L-DNA is chemically compatible with the D-form of DNA, so that chimeric molecules can be synthesized. We take advantage of the characteristics of L-DNA toward the establishment of a universal microarray that permits the analysis of different kinds of molecular diagnostic information in a single experiment on a single platform, in various combinations. Typical results for the measurement of transcript level variations, genotypic differences and DNA-protein interactions are presented. However, on the basis of the characteristic features of L-DNA, also other applications of this molecule type are discussed.