Dr.

Burger-Kentischer, Anke

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Immune cell-supplemented human skin model for studying fungal infections

2017 , Kühbacher, Andreas , Sohn, Kai , Burger-Kentischer, Anke , Rupp, Steffen

Human skin is a niche for various fungal species which either colonize the surface of this tissue as commensals or, primarily under conditions of immunosuppression, invade the skin and cause infection. Here we present a method for generation of a human in vitro skin model supplemented with immune cells of choice. This model represents a complex yet amenable tool to study molecular mechanisms of host-fungi interactions at human skin.

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3D-tissue model for herpes simplex virus-1 infections

2013 , Hogk, I. , Rupp, S. , Burger-Kentischer, A.

Infection with herpes simplex virus type 1 (HSV-1) causes the most common skin disease. Various test systems have been established to recapitulate this cyclical pathway of productive infection, latency, and reactivation. Most studies of latency and reactivation are conducted in animal models. However, the small number of neurons which harbor the viral genome, the complexity of the in vivo setting, and ethical constraints place limits on animal studies. So far, no in vitro model which resembles natural latency exists. Here, we describe the first in vitro HSV-1 infection model based on a human skin equivalent. The 3D infection model is generated using the human keratinocyte cell line HaCaT grown on a collagen substrate containing human primary fibroblasts and in addition a quiescently HSV-1 infected neuronal component.

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Controlled cell attachment, using plasma deposited polymer microstructures: A novel study of cells-substrate interactions

2006 , Sciarratta, V. , Sohn, K. , Burger-Kentischer, A. , Brunner, H. , Oehr, Christian

Several actual developments in medical therapy are focussing on the potential of a surface-dependent selection 1 and proliferation of special cell types. For an improvement of biocompatibility it is interesting to understand the interactions between cells and material surfaces in order to create devices with the respective characteristics. In this paper the possibility to isolate three different tumor cells lines are studied: HEK293, RINm5f and KYM-1. A procedure by means of plasma polymerization is demonstrated to create hydrophilic microstructures (precursor: AAc) on a hydrophobic (precursor: CHF3) substrate and shown to effectively select RINm5f cells. Moreover, by studying the interaction between culture media and deposited polymers, it is shown that Ca2+ ions and protein adsorption play a fundamental role in the cell-substrate adhesion and proliferation.

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