Fraunhofer-Institut für Produktionsanlagen und Konstruktionstechnik IPK

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  • Publication
    Development of a versatile and continuously operating cell disruption device
    ( 2013)
    Uhlmann, Eckart
    ;
    Oberschmidt, Dirk
    ;
    Spielvogel, Anja
    ;
    Herms, Katrin
    ;
    Polte, Mitchel
    ;
    Polte, Julian
    ;
    Dumke, A.
    Cell disruption is a recurrent unit operation in biotechnology. Interesting biotechnological products like proteins, lipids or biopolymers are synthesized intracellularly and are often not secreted. Furthermore, cell-free biotechnology uses defined fractions of the cytoplasm for in vitro protein synthesis. Bacteria, yeast, algae and filamentous fungi are surrounded by rigid cell walls that have to be disrupted by physical, chemical or mechanical methods in order to retain the valuable cell content. High pressure homogenization is a widely used procedure to disrupt cells and it has been applied to bacteria, algae and yeast. However, the mode of cell disruption has not been fully elucidated and performance is not predictable, thus time consuming iterative cycles are always necessary to define the best parameters for each microorganism, chemical environment and the corresponding product. Therefore, physical parameters of different biological systems were analyzed and boundary conditions defined in order to construct an adjustable disruption device to allow economical efficient, predictable and adjustable cell disruption processes.
  • Publication
    Tailored microfluidic chips for biotechnology
    ( 2013)
    Uhlmann, Eckart
    ;
    Mahr, Frederik
    ;
    Hein, Christoph
    ;
    Herms, Katrin
    ;
    Oberschmidt, Dirk
    Thermal damages of a workpiece are a limiting factor regarding the performance of grinding processes. The thermal load can be minimized when grinding with a reduced contact area between grinding wheel and workpiece. In this paper an experimental setup and machining strategy for the manufacturing of micro patterned grinding wheels are presented. Furthermore the results of grinding experiments with the use of micro patterned grinding wheels are shown.