Stetciura, Inna Y.Inna Y.StetciuraYashchenok, AlexeyAlexeyYashchenokMasic, AdmirAdmirMasicLyubin, Evgeny V.Evgeny V.LyubinInozemtseva, Olga A.Olga A.InozemtsevaDrozdova, Maria G.Maria G.DrozdovaMarkvichova, Elena A.Elena A.MarkvichovaKhlebtsov, Boris N.Boris N.KhlebtsovFedyanin, Andrey A.Andrey A.FedyaninSukhorukov, Gleb B.Gleb B.SukhorukovGorin, Dmitry A.Dmitry A.GorinVolodkin, DimitryDimitryVolodkin2022-03-052022-03-052015https://publica.fraunhofer.de/handle/publica/24173210.1039/c5an00392J2-s2.0-84951826091Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.en543journal article