CC BY-NC 4.0Vora, Kunj HimanshuKunj HimanshuVoraKordas, NorbertNorbertKordasSeidl, KarstenKarstenSeidl2025-11-122025-11-122024https://publica.fraunhofer.de/handle/publica/499216https://doi.org/10.24406/publica-629710.1016/j.sbsr.2024.10071510.24406/publica-62972-s2.0-85210534928This work describes the simulation, design, and fabrication of an inkjet-printed impedance biosensor for uromodulin which is a urine biomarker for kidney disease. The interdigitated electrodes (IDE) were fabricated through inkjet printing of silver ink on polyethylene terephthalate (PET) substrate and subsequently passivated with SU-8 for non-faradaic measurements. We functionalized the passivated IDE with biotin-streptavidin complex and uromodulin antibody to detect uromodulin (6.75 μg/ml-100 μg/ml). The measured impedance due to uromodulin immobilized from artificial urine samples showed a 27.4 % change in impedance magnitude per μg/ml of uromodulin at 150 Hz (log scale). The limit of detection achieved is 25 ng/ml. The sensor demonstrated specificity to uromodulin when measured against albumin, which is another protein biomarker present in the urine. These results highlight the possibility of implementing non-faradaic impedance measurements using a passivated IDE as the sensor element. It also promotes the sensor fabrication method of inkjet printing. The sensor concept shows the implementation potential of IDE as a single-use-sensor for point-of-care applications.entrueBiosensorImpedance spectroscopyInkjet-printingInterdigitated electrodesKidney-diseaseNon-faradaicSingle-use-sensorSU-8Uromodulinjournal article