Sellrie, F.F.SellrieLenz, C.C.LenzAndersson, A.A.AnderssonWilhelmsson, L.M.L.M.WilhelmssonSchenk, J.A.J.A.Schenk2022-03-052022-03-052014https://publica.fraunhofer.de/handle/publica/24810410.1016/j.talanta.2014.02.046We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA.en543journal article