Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus

Schmid, B.B.SchmidHolst, B.B.HolstPoulsen, U.U.PoulsenJørring, I.I.JørringClausen, C.C.ClausenRasmussen, M.M.RasmussenMau-Holzmann, U.A.U.A.Mau-HolzmannSteeg, R.R.SteegNuthall, H.H.NuthallEbneth, A.A.EbnethCabrera-Socorro, A.A.Cabrera-Socorro2022-03-062022-03-062021https://publica.fraunhofer.de/handle/publica/26821110.1016/j.scr.2021.102240Neurog2 is the gene encoding the neuronal transcription factor NGN2, which can convert stem cells into functional neurons in a fast and efficient way. Here we report the generation of two iPS cell lines, where DOX inducible constructs of neurog2 either without or with T2A-eGFP were inserted into the safe-site locus AAVS1. These iPS cell lines, BIONi010-C-13 and BIONi010-C-15, respectively, stay pluripotent without DOX but differentiate to (GFP positive) neurons when DOX is added without the need of differentiation factors.enGeneration of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locusjournal article