Steffen, J.J.SteffenNickisch-Rosenegk, M. vonM. vonNickisch-RosenegkBier, F.F.F.F.Bier2022-03-032022-03-032005https://publica.fraunhofer.de/handle/publica/20905110.1039/b501091hAn artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated.en610620540journal article