Sutiono, S.S.SutionoSatzinger, K.K.SatzingerPick, A.A.PickCarsten, J.J.CarstenSieber, V.V.Sieber2022-03-062022-03-062019https://publica.fraunhofer.de/handle/publica/26017510.1039/c9ra06251cPyruvate decarboxylase (PDC) is a key enzyme for the production of ethanol at high temperatures and for cell-free butanol synthesis. Thermostable, organic solvent stable PDC was evolved from bacterial PDCs. The new variant shows >1500-fold-improved half-life at 75 °C and >5000-fold-increased half-life in the presence of 9 vol% butanol at 50 °C.en660610620To beat the heat - engineering of the most thermostable pyruvate decarboxylase to datejournal article