Hiripi, L.L.HiripiMakovics, F.F.MakovicsHalter, R.R.HalterBaranyi, M.M.BaranyiPaul, D.D.PaulCarnwath, J.J.CarnwathBösze, Z.Z.BöszeNiemann, H.H.Niemann2022-03-032022-03-032003https://publica.fraunhofer.de/handle/publica/20401710.1089/104454903321112488Human clotting factor VIII is probably the largest protein to be expressed to date in the mammary gland of a transgenic animal, and it requires extensive posttranslational modification to achieve full biological activity. The mammary gland specific construct mWAP-hFVIII-MT-I was injected into the pronuclei of rabbit zygotes, and three transgenic offspring were obtained. Founder 385 showed germ-line transmission of a single integrated copy, and a homozygous line was established from this animal. The rhFVIII was transcribed and translated exclusively in the mammary gland. The activity of rhFVIII in the rabbit milk ranged from 5 to 8% of that found in normal human plasma. Results indicate the suitability of the transgenic rabbit mammary gland for rhFVIII production.enmammary glandhuman blood clotting factor VIIItransgenic animal615610620572journal article