LNP Synthesis Chip with in-situ Size Monitoring

The integration of a Low Aspect Ratio Laminar Mixer (LARLM) with inline dynamic light scattering (inline DLS) for the first time offers controlled and continuous lipid nanoparticle preparation with in situ size monitoring. The LARLM, a 3D micromixer fabricated by two-photon polymerisation (2PP), ensures that the aqueous phase flow completely envelops the organic phase flow, creating a thin and uniform layer with a thickness of down to 5 μ m. The thickness of the layer determining particle size is controlled. Inline DLS could prove the continuous production of lipid nanoparticles (LNPs) while their size was tunable between 60nm and 160 nm. Such stable and tunable process will ultimately contribute to better results in the formulation of mRNA drugs.