Cell transport using piezoelectric micro diaphragm pumps

New developments enable far-reaching cell culture research. However, many set-ups require manual fluid handling or bulky pump systems. The use of piezoelectric micropumps can enable further miniaturization and cost-efficient disposables, provided that cells are not damaged during transport. This work is first to investigate the impact of micropumps with flap valves on transported mammalian cells. The 5x5 mm² silicon pump with 20 µm stroke height impacts cells causing 35.9 % dead cells compared to 5 % in the control sample. The larger steel pump has less impact, especially with adapted, soft actuation. Further evaluation and the design of adapted pumps can potentially improve cell culture and in vitro cell test applications.