A Novel IMFET Biosensor Strategy for Interleukin-10 Quantification for Early Screening Heart Failure Disease in Saliva

We propose a salivary Interleukin-10 detection strategy as part of an easily integrable Lab-on-Chip and Point-of-Care IMFET for multi-detection Heart Failure (HF) biomarkers. Our developed IMFET showed good linearity between increasing IL-10 concentration and the charge transfer resistance in both standard solutions and real saliva samples with a LOD of 0.02 pg mL-1. Moreover, the cross-selectivity study showed that the developed IMFET was highly selective towards IL-10 against other HF biomarkers. The precision of our IMFET was also evaluated, and the difference between the determined IL-10 concentration using the standard addition method and its expected one is<20 %.