Voltammetric analysis of DNA films immobilized on gold film electrodes carrying single base pair mismatches

The application of electrochemical techniques for DNA detection is motivated by their potential to detect hybridisation events in a more rapid, simplistic and cost-effective manner compared to conventional optical assays. Here, we analyzed DNA films immobilized onto gold film electrodes containing single base pair mismatches and covalently attached methylene blue (MB) by means of cyclic (CV) and differential pulse voltammetry (DPV) and determined the rate constant for the electron transfer reaction between MB moiety and electrode for different types of mismatches. ©2007 IEEE.