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Title
Zweikammerkultivierungssystem mit elastischer, poroeser Membran zur uniaxialen mechanischen Stimulation von Epithelzellen und Epithelzellverbaenden fuer die Testung endogener und exogener Faktoren
Date Issued
2009
Author(s)
Kiesow, A.
Schwan, S.
Bartling, B.
Simm, A.
Patent No
102009057698
Abstract
DE 102009057698 A1 UPAB: 20110621 NOVELTY - The bioreactor for multi-dimensional, mechanical stimulation of cells, cell complexes and/or cell tissue, comprises a first and a second reactor part volumes (3, 4) that are separated from each other through a carrier membrane (2) on which the cell, the cell complexes and the cell tissue are immobilizable, and a microprocessor for static controlling and/or for dynamic controlling by feed back of the measuring device for the detection of the membrane deformation. The second reactor part volume is filled with a gas atmosphere. DETAILED DESCRIPTION - The bioreactor for multi-dimensional, mechanical stimulation of cells, cell complexes and/or cell tissue, comprises a first and a second reactor part volumes (3, 4) that are separated from each other through a carrier membrane (2) on which the cell, the cell complexes and the cell tissue are immobilizable, and a microprocessor for static controlling and/or for dynamic controlling by feed back of the measuring device for the detection of the membrane deformation. The second reactor part volume is filled with a gas atmosphere. The first reactor part volume is partially filled with a fluid cell culture medium. The carrier membrane is mechanically workable through designing an external mechanical field size. The pore has an average diameter of 300-500 nm. The average pore density is 5x 107-5x 108 pores/cm2 of the surface of the carrier membrane. The carrier membrane consists of rubber, natural rubber, silicon rubber and/or protein such as elastin. The carrier membrane has a thickness of 50-200 mu m and an elastic breaking strain of 20-100% and is formable through a pneumatic and/or hydraulic control device that stands in direct fluidic connection with the first and/or second reactor part volume. The control device is implemented as two magnet valves (6, 7) and/or as a reciprocating piston. The first reactor part volume and/or the second reactor part volume disposes over a sterile filter (5) for pressure balance. The first and/or the second reactor part volume disposes over a nozzle (8) in which the respective reactor volume is bringable over an external material. The first reactor part volume comprises a servo-valve with which a wetting of the carrier membrane is possible. The first reactor part volume and/or the second reactor part volume comprises a measuring device for the detection of the membrane deformation. The measuring device is a combination of LEDs, a charge-coupled device-array, a laser and/or detectors, and a capacitive measuring device such as two flat electrodes that are arranged in first and second reactor part volume, and/or a mechanical measuring device. The type of the cell is lung epithelial cells, vessel epithelial cells, intestinal epithelial cells and/or main epithelial cells. An INDEPENDENT CLAIM is included for a method for multi-dimensional, mechanical stimulation of cells, cell complexes and/or cell tissue. USE - Bioreactor useful for multi-dimensional, mechanical stimulation and analysis of cells, cell complexes and/or cell tissue. ADVANTAGE - The bioreactor ensures simple and efficient multi-dimensional, mechanical stimulation and analysis of cells, cell complexes and/or cell tissue.
Language
de
Institute
Patenprio
DE 102009057698 A: 20091203