Vorrichtung und Verfahren zur Konditionierung biologischer Zellen

(A1) Eine Konditionierungsvorrichtung (100) fuer biologische Zellen umfasst eine Halteeinrichtung (10), die zur Halterung von mindestens einer biologischen Zelle (1) in einem Haltebereich (11) eingerichtet ist, und eine Kontakteinrichtung (20), die zur Halterung von mindestens einer Konditionierungsprobe (3) und zur Bewegung der mindestens einen Konditionierungsprobe (3) zur Halteeinrichtung (10) derart eingerichtet ist, dass die mindestens eine Konditionierungsprobe (3) die mindestens eine biologische Zelle (1) beruehrt, wobei die Kontakteinrichtung (20) mindestens zwei mechanische Traegerelemente (21, 22) aufweist, die aus verschiedenen Raumrichtungen zur Halteeinrichtung (10) beweglich sind. Es werden auch Verfahren zur Konditionierung, insbesondere Praegungen, oder Differenzierung von biologischen Zellen und Anwendungen der Konditionierungsvorrichtung beschrieben.

DE 102007046516 A1 UPAB: 20090428 NOVELTY - The device for conditioning such as manipulation, stimulation, embossing and/or differentiation of biological cells e.g. animal or human cells, comprises a device (10) for holding a biological cell (1) in a holding area (11) in a contactless manner, and a contact device (20) for holding conditioning sample (3) and for moving the conditioning sample to the holding device in such a manner that the conditioning sample touches the biological cell. The contact device comprises ten mechanical carrier elements (21, 22), which are movable in different spatial directions for holding device. DETAILED DESCRIPTION - The device for conditioning such as manipulation, stimulation, embossing and/or differentiation of biological cells e.g. animal or human cells, comprises a device (10) for holding a biological cell (1) in a holding area (11) in a contactless manner, and a contact device (20) for holding conditioning sample (3) and for moving the conditioning sample to the holding device in such a manner that the conditioning sample touches the biological cell. The contact device comprises ten mechanical carrier elements (21, 22), which are movable in different spatial directions for holding device, and a holding surface, at which the biological cell is positionable. The holding surface is a mechanical holding element, and comprises a boundary surface between two cultivation phases and/or a biocompatible coating, which is biochemically ineffective for the biological cell. The holding device is arranged in a container for the reception of a liquid cultivation medium. The container has an interior volume, which is larger than the quadruple volume of the biological cell. The container comprises a bearing, in which one of the mechanical carrier elements or the mechanical holding element is adjustable, a fluid channel, by which a fluid connection is made with an environment of the container, a sensor for detecting a physical and/or chemical size in the container, and/or optical element for lighting and/or optical analysis in the container. One of the carrier elements has rod-shape and comprises a stamp surface for the reception of the conditioning sample, where the stamp surface is smaller than the surface of the biological cell, has a concave, convex or plane form, and comprises a micro-structuring and/or a test coating from biologically effective molecules. The conditioning device includes a driving device for moving one of the carrier elements to or from the holding area. The driving device contains a piezoelectric drive. The carrier elements are intended for a radially multilateral contact of the biological cell in the holding device. An INDEPENDENT CLAIM is included for a conditioning procedure for analyzing the interaction of a biological cell with a conditioning sample using a conditioning device. USE - Device for conditioning such as manipulation, stimulation, embossing and/or differentiation of biological cells e.g. animal or human cells (claimed). ADVANTAGE - The device ensures accurate, improved and reliable manipulation or analysis of biological cells e.g. animal/human cells.