Polypeptid-Tag's fuer den Nachweis und die Aufreinigung von Polypeptiden

Die vorliegende Erfindung betrifft Polypeptid-Tags, die zur Isolierung, zur Aufreinigung sowie zum Nachweis von Polypeptiden verwendet werden koennen. Die vorliegende Erfindung betrifft insbesondere Expressionsvektoren, die ein Fusionspolypeptid codieren, das ein tag54-Epitop und ein gewuenschtes Polypeptid umfasst, sowie die Verwendung derartiger Expressionsvektoren zur Expression der Fusionspolypeptide. Ferner betrifft die Erfindung Antikoerper gegen die mit dem beschriebenen Polypeptid-Tag generierten Fusionspeptide, deren Verwendung zur Isolierung, zur Aufreinigung sowie zum Nachweis der Fusionspeptide, sowie die zu diesem Zweck geeigneten Kits.

EP 1092769 A UPAB: 20010522 NOVELTY - Polypeptide with tag sequence (I) or a sequence derived from (I) by replacement of one or more amino acids (aa), provided the three-dimensional/tertiary structure remains essentially unchanged, is new. DETAILED DESCRIPTION - Polypeptide having the tag sequence KDWEHL (I) or a sequence derived from it by replacement of one or more amino acids (aa), provided the three-dimensional/tertiary structure remains essentially unchanged. INDEPENDENT CLAIMS are also included for the following: (1) expression vector encoding a fusion protein (FP) of a target polypeptide (II) and (I); (2) FP encoded by the vector of (1); (3) antibodies (Ab), or their fragments, directed against (I); and (4) kit containing the vector of (1) and optionally Ab. USE - (I) are used for isolation and/or detection of fusion polypeptides that contain (I), by reaction with (I)-specific antibodies (Ab). Ab are used for isolation, purification and/or detection of polypeptides (also for determination of molecular weight, cell localization, post-translational modification and interaction with other factors), and for diagnosis of infection by tobacco mosaic virus (TMV). ADVANTAGE - (I) is recognized by very specific, high-affinity antibodies that do not cross-react significantly with other cellular proteins (bacterial or eukaryotic).